ARG81241

Mouse MMP9 ELISA Kit

Mouse MMP9 ELISA Kit for ELISA and Mouse

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Overview

Product Description

ARG81241 Mouse MMP9 ELISA Kit is an Enzyme Immunoassay Kit for the quantification of Mouse MMP9 in serum, plasma and cell culture supernatants.

Tested Reactivity Ms
Tested Application ELISA
Target Name MMP9
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 156.25 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 312.5 - 20000 pg/ml
Sample Volume 100 µl
Alternate Names Matrix metalloproteinase-9; 92 kDa gelatinase; MMP-9; Gelatinase B; GELB; CLG4B; MANDP2; EC 3.4.24.35; 92 kDa type IV collagenase

Application Instructions

Assay Time ~ 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17395 Mouse MMP9

Swiss-port # P41245 Mouse Matrix metalloproteinase-9

Gene Symbol Mmp9
Gene Full Name matrix metallopeptidase 9
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. [UniProt]
Highlight Related products:
MMP9 antibodies; MMP9 ELISA Kits; MMP9 Duos / Panels;
Related news:
New MMP7 (total or active) ELISA kits are released
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.

Specific References

Qilongtian ameliorate bleomycin-induced pulmonary fibrosis in mice via inhibiting IL-17 signal pathway

ELISA / Mouse

Qiang Zhang et al.
Sci Rep.,  (2023)

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Title Download Link
ARG81241 Mouse MMP9 ELISA Kit User's manual download icon Download