ARG81785

Mouse MPO / Myeloperoxidase ELISA Kit

Mouse MPO / Myeloperoxidase ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81785-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81785-002 Standard 2 X 10 ng/vial 4°C
ARG81785-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81785-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81785-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81785-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81785-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81785-008 25X Wash buffer 20 ml 4°C
ARG81785-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81785-010 STOP solution 10 ml (Ready to use) 4°C
ARG81785-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81785 Mouse MPO / Myeloperoxidase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MPO / Myeloperoxidase in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MPO / Myeloperoxidase
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 7.2%
Alternate Names MPO; Myeloperoxidase; EC 1.11.2.2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17523 Mouse MPO

Swiss-port # P11247 Mouse Myeloperoxidase

Gene Symbol MPO
Gene Full Name myeloperoxidase
Background Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Function Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt]
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Title Download Link
ARG81785 Mouse MPO / Myeloperoxidase ELISA Kit User manual download icon Download