ARG81893

Mouse Neuropilin 1 ELISA Kit

Mouse Neuropilin 1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81893-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81893-002 Standard 2 X 50 ng/vial 4°C
ARG81893-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81893-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81893-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81893-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81893-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81893-008 25X Wash buffer 20 ml 4°C
ARG81893-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81893-010 STOP solution 10 ml (Ready to use) 4°C
ARG81893-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81893 Mouse Neuropilin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Neuropilin 1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Neuropilin 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 390 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 780 - 50000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.2%; Inter-Assay CV: 7.7%
Alternate Names BDCA4; VEGF165R; CD antigen CD304; Neuropilin-1; Vascular endothelial cell growth factor 165 receptor; CD304; NRP; NP1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18186 Mouse NRP1

Swiss-port # P97333 Mouse Neuropilin-1

Gene Symbol NRP1
Gene Full Name neuropilin 1
Background This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
Function The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.

The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. [UniProt]
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