ARG81927

Mouse Neutrophil Elastase ELISA Kit

Mouse Neutrophil Elastase ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81927-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81927-002 Standard 2 X 10 ng/vial 4°C
ARG81927-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81927-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81927-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81927-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81927-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81927-008 25X Wash buffer 20 ml 4°C
ARG81927-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81927-010 STOP solution 10 ml (Ready to use) 4°C
ARG81927-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81927 Mouse Neutrophil Elastase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Neutrophil Elastase in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Neutrophil Elastase
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.0%; Inter-Assay CV: 7.3%
Alternate Names Neutrophil elastase; HNE; EC 3.4.21.37; Medullasin; NE; ELA2; GE; Bone marrow serine protease; SCN1; PMN-E; Human leukocyte elastase; HLE; Elastase-2; PMN elastase

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 50701 Mouse ELANE

Swiss-port # Q3UP87 Mouse Neutrophil elastase

Gene Symbol ELANE
Gene Full Name elastase, neutrophil expressed
Background Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]
Function Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. [UniProt]
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Title Download Link
ARG81927 Mouse Neutrophil Elastase ELISA Kit User manual download icon Download