ARG70316
Mouse PLGF recombinant protein (Fc-His-tagged, C-ter)
Mouse PLGF recombinant protein (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Mouse PLGF recombinant protein. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | Binding, SDS-PAGE |
| Target Name | PLGF |
| Species | Mouse |
| A.A. Sequence | Met1 - Pro158 of Mouse PLGF (NP_032853.1) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | PGFL; PlGF; PlGF-2; SHGC-10760; Placenta growth factor; D12S1900; PLGF |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGFR1 at 500ng/ml (100 µl/well) can bind Recombinant Mouse PLGF with a linear range of 12-49 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 87% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PGF |
| Gene Full Name | placental growth factor |
| Background | This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| Function | Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. [UniProt] |
| Cellular Localization | Secreted. Note=The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | N-glycosylated. [UniProt] |
