ARG70316

Mouse PLGF recombinant protein (Fc-His-tagged, C-ter)

Mouse PLGF recombinant protein (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse

Overview

Product Description HEK293 expressed, Fc-His-tagged (C-ter) Mouse PLGF recombinant protein.
Tested Reactivity Ms
Tested Application Binding, SDS-PAGE
Target Name PLGF
Species Mouse
A.A. Sequence Met1 - Pro158 of Mouse PLGF (NP_032853.1) with an Fc-6X His tag at the C-terminus.
Expression System HEK293
Alternate Names PGFL; PlGF; PlGF-2; SHGC-10760; Placenta growth factor; D12S1900; PLGF

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGFR1 at 500ng/ml (100 µl/well) can bind Recombinant Mouse PLGF with a linear range of 12-49 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test.
Purity > 87% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18654 Mouse PGF

Swiss-port # P49764 Mouse Placenta growth factor

Gene Symbol PGF
Gene Full Name placental growth factor
Background This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
Function Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. [UniProt]
Cellular Localization Secreted. Note=The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. [UniProt]
Calculated MW 25 kDa
PTM N-glycosylated. [UniProt]