ARG81756

Mouse RBP4 ELISA Kit

Mouse RBP4 ELISA Kit for ELISA and Mouse

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Component

Cat No Component Name Package Temp
ARG81756-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81756-002 Standard 2 X 20 ng/vial 4°C
ARG81756-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81756-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81756-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81756-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81756-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81756-008 25X Wash buffer 20 ml 4°C
ARG81756-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81756-010 STOP solution 10 ml (Ready to use) 4°C
ARG81756-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81756 Mouse RBP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse RBP4 in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name RBP4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.2%
Alternate Names Plasma retinol-binding protein; MCOPCB10; 1-176; RDCCAS; RBP; Retinol-binding protein 4; PRBP; 1-182; 1-179; 1-181

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19662 Mouse RBP4

Swiss-port # Q00724 Mouse Retinol-binding protein 4

Gene Symbol RBP4
Gene Full Name retinol binding protein 4, plasma
Background This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]
Function Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. [UniProt]
Highlight Related products:
RBP4 antibodies; RBP4 ELISA Kits; RBP4 Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Specific References

A novel mouse model of heart failure with preserved ejection fraction after chronic kidney disease induced by retinol through JAK/STAT pathway

ELISA / Mouse / Serum

Bowen Liu et al.
Int J Biol Sci.,  (2023)

publication_link

 

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Title Download Link
ARG81756 Mouse RBP4 ELISA Kit User's manual download icon Download