ARG81310
Mouse/Rat HMGB1 ELISA Kit
Mouse/Rat HMGB1 ELISA Kit for ELISA and Mouse,Rat
Publication34
Overview
| Product Description | ARG81310 Mouse/Rat HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse/Rat HMGB1 in plasma, cell culture supernatants and cell/tissue lysate samples. |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | ELISA |
| Specificity | Cross-react to mouse HMGB2 is less than 5%. |
| Target Name | HMGB1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.3 ng/ml |
| Sample Type | Plasma, cell culture supernatants, Bronchoalveolar lavage fluid (BALF) and cell/tissue lysate samples. |
| Standard Range | 0.3125 - 20 ng/ml |
| Sample Volume | 100 µl |
| Precision | < 5.2% |
| Alternate Names | HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3 |
Application Instructions
| Assay Time | Overnight |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Hmgb1 |
| Gene Full Name | high mobility group box 1 |
| Background | HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015] |
| Function | HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt] |
| Highlight | Related products: HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 Duos / Panels; HMGB1 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 Total solution for HMGB1 research HMGB1 in inflammation Inflammatory Cytokines HMGB1 ELISA Kit for your research Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit New HMGB1 neutralizing antibody is released Detecting exosomal HMGB1 for ICD research Related poster download: HMGB1 Pathway.pdf New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460). Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity). Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so). Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity). In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex. |
Specific References
A mechanism of IL-34-induced resistance against cytotoxic anti-cancer therapies such as radiation by X-ray and chemotherapy by Oxaliplatin
ELISA / Mouse
Hydrogen alleviated cognitive impairment and blood‒brain barrier damage in sepsis-associated encephalopathy by regulating ABC efflux transporters in a PPARα-dependent manner
ELISA / Mouse
Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage
ELSIA / Mouse
Continuous infiltration of small peritoneal macrophages in the mouse peritoneum through CCR2-dependent and -independent routes during fibrosis and mesothelioma development induced by a multiwalled carbon nanotube, MWNT-7
ELISA / Mouse / Cell culture supernatant
Chemokine-derived oncolytic peptide induces immunogenic cancer cell death and significantly suppresses tumor growth
ELISA / Mouse / Cell culture supernatant
Enhanced Therapeutic Efficacy of Combining Losartan and Chemo-Immunotherapy for Triple Negative Breast Cancer
ELISA / Mouse / Culture supernatant
Radiation therapy enhances systemic antitumor efficacy in PD-L1 therapy regardless of sequence of radiation in murine osteosarcoma
ELISA / Mouse / Culture supernatant
Perfluorooctyl bromide nanoemulsions holding MnO2 nanoparticles with dual-modality imaging and glutathione depletion enhanced HIFU-eliciting tumor immunogenic cell death
ELISA / Mouse / Cell culture supernatant
High Dose Local Photon Irradiation Is Crucial in Anti-CTLA-4 Antibody Therapy to Enhance the Abscopal Response in a Murine Pancreatic Carcinoma Model
ELISA / Mouse / Cell culture supernatant
Assessment of the toxicity and carcinogenicity of double-walled carbon nanotubes in the rat lung after intratracheal instillation: a two-year study
ELISA / Rat / Pleural lavage fuid
Regulatory mechanisms of HMGB1 and its receptors in polycystic ovary syndrome-driven gravid uterine inflammation
ELISA / Rat
Cutaneous ischemia-reperfusion injury is exacerbated by IL-36 receptor antagonist deficiency
ELISA / Mouse / Serum
Distinct roles of HMGB1 in the regulation of P glycoprotein expression in the liver and kidney of mice with lipopolysaccharide-induced inflammation
ELISA / Mouse / Plasma; Tissue lysate
Early alveolar epithelial cell necrosis is a potential driver of COVID-19-induced acute respiratory distress syndrome
ELISA / Mouse/Cell culture supernatant
Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumors
ELISA / Mouse / Serum , Cell culture supernatant
Distinct roles of HMGB1 in the regulation of P‑glycoprotein expression in the liver and kidney of mice with lipopolysaccharide‑induced inflammation
ELISA / Mouse / Plasma; Tissue lysate
Spatiotemporal depletion of tumor-associated immune checkpoint PD-L1 with near-infrared photoimmunotherapy promotes antitumor immunity
ELISA / Mouse / Cell culture supernatant
Sodium Butyrate Alleviates Intestinal Inflammation in Mice with Necrotizing Enterocolitis
ELISA / Mouse / Tissue lysate
Glycyrrhizin ameliorates melanoma cell extravasation into mouse lungs by regulating signal transduction through HMGB1 and its receptors
ELISA / Mouse
Homologous-targeted and tumor microenvironment-activated hydroxyl radical nanogenerator for enhanced chemoimmunotherapy of non-small cell lung cancer
ELISA / Mouse Cell culture supernatant
The Efficacy of Phage Therapy in a Murine Model of Pseudomonas aeruginosa Pneumonia and Sepsis
ELISA / Mouse
High-density lipoprotein modulates tumor-associated macrophage for chemoimmunotherapy of hepatocellular carcinoma.
ELISA / Mouse / Cell culture medium
Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity.
ELISA / Mouse
Clara cell 16 KDa protein mitigates house dust mite-induced airway inflammation and damage via regulating airway epithelial cell apoptosis in a manner dependent on HMGB1-mediated signaling inhibition.
ELISA / Mouse / BALF
Combination of Cisplatin and Irradiation Induces Immunogenic Cell Death and Potentiates Postirradiation Anti-PD-1 Treatment Efficacy in Urothelial Carcinoma.
ELISA / Mouse / Cell culture supernatant
Molecular hydrogen alleviates brain injury and cognitive impairment in a chronic sequelae model of murine polymicrobial sepsis.
ELISA / Mouse / Tissue lysate
Tumor-derived HMGB1 induces CD62L dim neutrophil polarization and promotes lung metastasis in triple-negative breast cancer.
ELISA / Mouse / Serum and culture medium
Carrier-free nanoassembly of doxorubicin prodrug and siRNA for combinationally inducing immunogenic cell death and reversing immunosuppression.
ELISA / Mouse / Cell culture supernatant
Comparison of Exosomes Derived from Non- and Gamma-Irradiated Melanoma Cancer Cells as a Potential Antigenic and Immunogenic Source for Dendritic Cell-Based Immunotherapeutic Vaccine.
ELISA / Mouse
Irradiation-induced osteocyte damage promotes HMGB1-mediated osteoclastogenesis in vitro.
ELISA / Mouse
Carbon ion irradiation enhances the antitumor efficacy of dual immune checkpoint blockade therapy both for local and distant sites in murine osteosarcoma.
ELISA / Mouse
Inhibition of HMGB1 Promotes Osseointegration under Hyperglycemic Condition through Improvement of BMSC Dysfunction.
ELISA / Rat
Syringeable immunotherapeutic nanogel reshapes tumor microenvironment and prevents tumor metastasis and recurrence.
ELISA / Mouse
Targeting HMGB1 by ethyl pyruvate ameliorates systemic lupus erythematosus and reverses the senescent phenotype of bone marrow-mesenchymal stem cells.
ELISA /
| Title | Download Link |
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| ARG81310 Mouse/Rat HMGB1 ELISA Kit User's manual |
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