Component

Overview

Product Description	ARG82522 Mouse SLIT2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse SLIT2 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity	Ms
Tested Application	ELISA
Target Name	SLIT2
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm.
Sensitivity	78 pg/ml
Sample Type	Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range	156 - 10000 pg/ml
Sample Volume	100 µl
Precision	Intra-Assay CV: 5.3% Inter-Assay CV: 6.9%
Alternate Names	Slit-2; Slit homolog 2 protein; SLIL3

Application Instructions

Assay Time	~ 5 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 20563 Mouse SLIT2 Swiss-port # Q9R1B9 Mouse Slit homolog 2 protein
Gene Symbol	SLIT2
Gene Full Name	slit guidance ligand 2
Background	This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function	Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. [UniProt]
Cellular Localization	Secreted. Note=The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated. [UniProt]
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