ARG82541

Mouse Semaphorin 6A ELISA Kit

Mouse Semaphorin 6A ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82541-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82541-002 Standard 2 X 10 ng/vial 4°C
ARG82541-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82541-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82541-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82541-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82541-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82541-008 25X Wash buffer 20 ml 4°C
ARG82541-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82541-010 STOP solution 10 ml (Ready to use) 4°C
ARG82541-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82541 Mouse Semaphorin 6A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Semaphorin 6A in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name Semaphorin 6A
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 50 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.8%
Inter-Assay CV: 6.7%
Alternate Names VIA; Semaphorin VIA; Semaphorin-6A; SEMAQ; Semaphorin-6A-1; SEMA; Sema VIA; SEMA6A-1; HT018; SEMA6A1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20358 Mouse SEMA6A

Swiss-port # O35464 Mouse Semaphorin-6A

Gene Symbol SEMA6A
Gene Full Name sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Background The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al., 2001 [PubMed 11242070]).[supplied by OMIM, Feb 2011]
Function Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
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Title Download Link
ARG82541 Mouse Semaphorin 6A ELISA Kit User manual download icon Download