ARG82088

Mouse TNFSF18 / GITRL ELISA Kit

Mouse TNFSF18 / GITRL ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82088-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82088-002 Standard 2 X 10 ng/vial 4°C
ARG82088-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82088-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82088-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82088-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82088-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82088-008 25X Wash buffer 20 ml 4°C
ARG82088-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82088-010 STOP solution 10 ml (Ready to use) 4°C
ARG82088-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82088 Mouse TNFSF18 / GITRL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TNFSF18 / GITRL in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name TNFSF18 / GITRL
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.4%; Inter-Assay CV: 6.9%
Alternate Names TL6; AITRL; Glucocorticoid-induced TNF-related ligand; Tumor necrosis factor ligand superfamily member 18; hGITRL; Activation-inducible TNF-related ligand; GITRL

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 240873 Mouse TNFSF18

Swiss-port # Q7TS55 Mouse Tumor necrosis factor ligand superfamily member 18

Gene Symbol TNFSF18
Gene Full Name tumor necrosis factor (ligand) superfamily, member 18
Background The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq, Jul 2008]
Function Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1. Promotes leukocyte adhesion to endothelial cells. Regulates migration of monocytes from the splenic reservoir to sites of inflammation (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type II membrane protein. [UniProt]
Highlight Related products:
TNFSF antibodies; TNFSF ELISA Kits; TNFSF Duos / Panels; TNFSF recombinant proteins;
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Title Download Link
ARG82088 Mouse TNFSF18 / GITRL ELISA Kit User's manual download icon Download