ARG82088
Mouse TNFSF18 / GITRL ELISA Kit
Mouse TNFSF18 / GITRL ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82088-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82088-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82088-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82088-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82088-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82088-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82088-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82088-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82088-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82088-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82088-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82088 Mouse TNFSF18 / GITRL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TNFSF18 / GITRL in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TNFSF18 / GITRL |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.4%; Inter-Assay CV: 6.9% |
| Alternate Names | TL6; AITRL; Glucocorticoid-induced TNF-related ligand; Tumor necrosis factor ligand superfamily member 18; hGITRL; Activation-inducible TNF-related ligand; GITRL |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q7TS55 Mouse Tumor necrosis factor ligand superfamily member 18 |
|---|---|
| Gene Symbol | TNFSF18 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 18 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1. Promotes leukocyte adhesion to endothelial cells. Regulates migration of monocytes from the splenic reservoir to sites of inflammation (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. [UniProt] |
| Highlight | Related products: TNFSF antibodies; TNFSF ELISA Kits; TNFSF Duos / Panels; TNFSF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG82088 Mouse TNFSF18 / GITRL ELISA Kit User's manual |
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