ARG81936
Mouse TYRO3 ELISA Kit
Mouse TYRO3 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81936-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81936-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81936-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81936-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81936-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81936-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81936-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81936-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81936-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81936-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81936-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81936 Mouse TYRO3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TYRO3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TYRO3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.9%; Inter-Assay CV: 7.5% |
| Alternate Names | Dtk; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY; Tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase DTK; RSE; Sky; Rek; Tyrosine-protein kinase TIF; BYK; Tif; EC 2.7.10.1; Tyrosine-protein kinase BYK; Etk-2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P55144 Mouse Tyrosine-protein kinase receptor TYRO3 |
|---|---|
| Gene Symbol | TYRO3 |
| Gene Full Name | TYRO3 protein tyrosine kinase |
| Background | The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses. [provided by RefSeq, May 2010] |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. [UniProt] |
| Highlight | Related products: TYRO3 antibodies; TYRO3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Autophosphorylated. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81936 Mouse TYRO3 ELISA Kit User's manual |
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