ARG81812

Mouse Thrombomodulin ELISA Kit

Mouse Thrombomodulin ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81812-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81812-002 Standard 2 X 10 ng/vial 4°C
ARG81812-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81812-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81812-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81812-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81812-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81812-008 25X Wash buffer 20 ml 4°C
ARG81812-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81812-010 STOP solution 10 ml (Ready to use) 4°C
ARG81812-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81812 Mouse Thrombomodulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Thrombomodulin in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Thrombomodulin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%; Inter-Assay CV: 6.3%
Alternate Names THRM; CD antigen CD141; THPH12; TM; Thrombomodulin; CD141; BDCA3; Fetomodulin; AHUS6

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21824 Mouse THBD

Swiss-port # P15306 Mouse Thrombomodulin

Gene Symbol THBD
Gene Full Name thrombomodulin
Background The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008]
Function Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. [UniProt]
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PTM N-glycosylated.

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. [UniProt]
Title Download Link
ARG81812 Mouse Thrombomodulin ELISA Kit User's manual download icon Download