ARG80207

Mouse VEGF ELISA Kit

Mouse VEGF ELISA Kit for ELISA and Mouse

Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80207-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80207-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80207-003 Standard diluent buffer 20 ml 4°C
ARG80207-004 Antibody conjugate concentrate 400 µl 4°C
ARG80207-005 Antibody diluent buffer 16 ml 4°C
ARG80207-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80207-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80207-008 20X Wash buffer 50 ml 4°C
ARG80207-009 TMB substrate 12ml 4°C (Protect from light)
ARG80207-010 STOP solution 12ml 4°C
ARG80207-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80207 Mouse VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse VEGF in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Mouse VEGF121 VEGF R2/Fc Chimera, VEGF165, VEGF165/PlGF
Target Name VEGF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision CV: <10%
Alternate Names MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22339 Mouse VEGFA

Swiss-port # Q00731 Mouse Vascular endothelial growth factor A

Gene Symbol Vegfa
Gene Full Name vascular endothelial growth factor A
Background This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq, Jul 2008]
Highlight Related products:
VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins;
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit

Specific References

NE/β2-AR Promotes Directional Migration of Colorectal Cancer Cells to the Liver by Inducing M2 Polarization of Kupffer Cells and Upregulating CXCL12 Expression

ELISA / Mouse

Xiaoyan Li et al.
Research Square,  (2022)

publication_link

 

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Title Download Link
ARG80207 Mouse VEGF ELISA Kit User's manual download icon Download