ARG63038
Mouse anti-Bovine IgG (Light Chain) antibody [IVA285-1]
Mouse anti-Bovine IgG (Light Chain) antibody [IVA285-1] for Flow cytometry,IHC-Frozen sections,Western blot and Bovine
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [IVA285-1] recognizes Bovine IgG (Light Chain) |
|---|---|
| Tested Reactivity | Bov |
| Tested Application | FACS, IHC-Fr, WB |
| Specificity | The clone IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | IVA285-1 |
| Isotype | IgG1 |
| Target Name | IgG (Light Chain) |
| Immunogen | Fraction of Bovine immunoglobulin. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Incubation: Overnight at 4°C. Sample preparation: Bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Application notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Under reducing condition. SDS-PAGE (12% separating gel). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: Bovine serum |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Immune System antibody |
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