ARG63045

Mouse anti-Human Kappa Light Chain antibody [MEM-09]

Mouse anti-Human Kappa Light Chain antibody [MEM-09] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-09] recognizes Human Kappa Light Chain
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-P, IP, WB
Specificity The clone MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa).
Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
Host Mouse
Clonality Monoclonal
Clone MEM-09
Isotype IgG1
Target Name Kappa Light Chain
Immunogen Crude thymus membrane fraction.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS5 µg/ml
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3514 Human IGKC

Background Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Research Area Immune System antibody

Clone References

Cell line-specific control of recombinant monoclonal antibody production by CHO cells.

WB / Chinese Hamster

O'Callaghan PM et al.
Biotechnol Bioeng.,  (2010)

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Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.

Horejsí V et al.
Folia Biol (Praha).,  (1986)

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