ARG82083

Mouse sCD40L ELISA Kit

Mouse sCD40L ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82083-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82083-002 Standard 2 X 10 ng/vial 4°C
ARG82083-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82083-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82083-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82083-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82083-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82083-008 25X Wash buffer 20 ml 4°C
ARG82083-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82083-010 STOP solution 10 ml (Ready to use) 4°C
ARG82083-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82083 Mouse sCD40L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse sCD40L in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name sCD40L
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.7%; Inter-Assay CV: 7.7%
Alternate Names TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21947 Mouse CD40LG

Swiss-port # P27548 Mouse CD40 ligand

Gene Symbol CD40LG
Gene Full Name CD40 ligand
Background The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Function Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt]
Cellular Localization Cell membrane; Single-pass type II membrane protein. Cell surface. CD40 ligand, soluble form: Secreted. [UniProt]
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CD40L antibodies; CD40L ELISA Kits;
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PTM The soluble form derives from the membrane form by proteolytic processing.

N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.

Not O-glycosylated. [UniProt]
Title Download Link
ARG82083 Mouse sCD40L ELISA Kit User's manual download icon Download