ARG82083
Mouse sCD40L ELISA Kit
Mouse sCD40L ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82083-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82083-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82083-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82083-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82083-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82083-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82083-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82083-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82083-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82083-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82083-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82083 Mouse sCD40L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse sCD40L in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | sCD40L |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7%; Inter-Assay CV: 7.7% |
| Alternate Names | TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD40LG |
| Gene Full Name | CD40 ligand |
| Background | The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008] |
| Function | Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Cell surface. CD40 ligand, soluble form: Secreted. [UniProt] |
| Highlight | Related products: CD40L antibodies; CD40L ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82083 Mouse sCD40L ELISA Kit User's manual |
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