ARG81849

Mouse uPAR ELISA Kit

Mouse uPAR ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81849-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81849-002 Standard 2 X 10 ng/vial 4°C
ARG81849-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81849-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81849-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81849-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81849-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81849-008 25X Wash buffer 20 ml 4°C
ARG81849-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81849-010 STOP solution 10 ml (Ready to use) 4°C
ARG81849-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81849 Mouse uPAR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse uPAR in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name uPAR
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.7%; Inter-Assay CV: 7.8%
Alternate Names Monocyte activation antigen Mo3; CD antigen CD87; uPAR; U-PAR; URKR; Urokinase plasminogen activator surface receptor; UPAR; CD87

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18793 Mouse PLAUR

Swiss-port # P35456 Mouse Urokinase plasminogen activator surface receptor

Gene Symbol PLAUR
Gene Full Name plasminogen activator, urokinase receptor
Background This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]
Function Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. [UniProt]
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Title Download Link
ARG81849 Mouse uPAR ELISA Kit User's manual download icon Download