ARG70212
Pig GM-CSF recombinant protein (Active) (His-tagged, N-ter)
Pig GM-CSF recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Pig GM-CSF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | GM-CSF |
| Species | Pig |
| A.A. Sequence | APTRP PSPVT RPWQH VDAIK EALSL LNNSN DTAAV MNETV DVVCE MFDPQ EPTCV QTRLN LYKQG LRGSL TRLKS PLTLL AKHYE QHCPL TEETS CETQS ITFKS FKDSL NKFLF TIPFD CWGPV KK with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is < 3 ng/ml. |
| Alternate Names | GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CSF2 |
|---|---|
| Gene Full Name | colony stimulating factor 2 (granulocyte-macrophage) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines |
