ARG82993

Porcine BDNF ELISA Kit

Porcine BDNF ELISA Kit for ELISA and Pig

Component

Cat No Component Name Package Temp
ARG82993-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82993-002 Standard 2 X 10 ng/vial 4°C
ARG82993-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82993-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82993-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82993-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82993-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82993-008 25X Wash buffer 20 ml 4°C
ARG82993-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82993-010 STOP solution 10 ml (Ready to use) 4°C
ARG82993-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82993 Porcine BDNF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human BDNF in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants
Tested Reactivity Pig
Tested Application ELISA
Specificity There is no detectable cross-reactivity with recombinant human CNTF, beta-NGF, NT-3, NT-4, recombinant mouse beta-NGF, NT-4 and recombinant rat beta-NGF.
Target Name BDNF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.2%
Inter-Assay CV: 7.6%
Alternate Names Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 397495 Pig BDNF

Swiss-port # P14082 Pig Brain-derived neurotrophic factor

Gene Symbol BDNF
Gene Full Name brain-derived neurotrophic factor
Background The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]
Function During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt]
Cellular Localization Secreted. Note: A proportion of BDNF is secreted as immature precursor (proBDNF). [UniProt]
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BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins;
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PTM The propeptide is N-glycosylated and glycosulfated.

Converted into mature BDNF by plasmin (PLG). [UniProt]
Title Download Link
ARG82993 Porcine BDNF ELISA Kit User manual download icon Download