ARG82286
Porcine VEGF ELISA Kit
Porcine VEGF ELISA Kit for ELISA and Pig
Overview
| Product Description | ARG82286 Porcine VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine VEGF in serum and plasma. |
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| Tested Reactivity | Pig |
| Tested Application | ELISA |
| Specificity | Not react with following recombinant porcine proteins: ApoAI, BMP1, BMP2, BMP4, BMP7, HGF, HSP27, IL1 beta, IL1 RA, IL2, I-2R, IL5, IL6, IL6R, IL8, IL10, IL12, IL13, IL15, IL17C, IL23, IFN gamma, MMP2, MMP9, PDGF AA, PDGF BB, PDGF AB, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI and TNF RII. |
| Target Name | VEGF |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 19.5 pg/ml |
| Sample Type | Serum and plasma. |
| Standard Range | 39 - 2500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4% Inter-Assay CV: 9% |
| Alternate Names | MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P49151 Pig Vascular endothelial growth factor A |
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| Gene Symbol | VEGFA |
| Gene Full Name | vascular endothelial growth factor A |
| Background | This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008] |
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [UniProt] |
| Cellular Localization | Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. [UniProt] |
| Highlight | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Related news: The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
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| ARG82286 Porcine VEGF ELISA Kit User maunla |
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