ARG81189
Rat Alpha-2-Macroglobulin ELISA Kit
Rat Alpha-2-Macroglobulin ELISA Kit for ELISA and Rat
Overview
| Product Description | ARG81189 Rat Alpha-2-Macroglobulin ELISA is an Enzyme Immunoassay kit for the quantification of Rat Alpha-2-Macroglobulin in serum, plasma, cell culture and urine. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | alpha-2-Macroglobulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | ~ 0.1 ng/ml |
| Sample Type | Serum, plasma, cell culture and urine. |
| Standard Range | 0.156 - 10 ng/ml |
| Sample Volume | 50 µl |
| Alternate Names | CPAMD5; Alpha-2-macroglobulin; S863-7; FWP007; Alpha-2-M; A2MD; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | A2m |
| Gene Full Name | alpha-2-macroglobulin |
| Background | Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008] |
| Function | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. [UniProt] |
| Highlight | Related products: alpha-2-Macroglobulin antibodies; alpha-2-Macroglobulin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
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| ARG81189 Rat Alpha-2-Macroglobulin ELISA Kit User's manual |
 Download
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