ARG81876

Rat BTC / Betacellulin ELISA Kit

Rat BTC / Betacellulin ELISA Kit for ELISA and Rat

publication_link Publication1

Component

Cat No Component Name Package Temp
ARG81876-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81876-002 Standard 2 X 10 ng/vial 4°C
ARG81876-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81876-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81876-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81876-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81876-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81876-008 25X Wash buffer 20 ml 4°C
ARG81876-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81876-010 STOP solution 10 ml (Ready to use) 4°C
ARG81876-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81876 Rat BTC / Betacellulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat BTC / Betacellulin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name BTC / Betacellulin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 3.9 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%; Inter-Assay CV: 6.6%
Alternate Names Probetacellulin; BTC

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol BTC
Gene Full Name betacellulin
Background The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. [provided by RefSeq, Jul 2008]
Function Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. [UniProt]
Highlight Related products:
Betacellulin antibodies; Betacellulin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Specific References

Betacellulin regulates peripheral nerve regeneration by affecting Schwann cell migration and axon elongation

ELISA / Rat

Yaxian Wang et al.
Mol Med.,  (2021)

publication_link

 

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Title Download Link
ARG81876 Rat BTC / Betacellulin ELISA Kit User's manual download icon Download