ARG80246

Rat CCL2 / MCP1 ELISA Kit

Rat CCL2 / MCP1 ELISA Kit for ELISA and Rat

Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit

Component

Cat No Component Name Package Temp
ARG80246-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80246-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80246-003 Standard diluent buffer 20 ml 4°C
ARG80246-004 Antibody conjugate concentrate 400 µl 4°C
ARG80246-005 Antibody diluent buffer 16 ml 4°C
ARG80246-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80246-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80246-008 20X Wash buffer 50 ml 4°C
ARG80246-009 TMB substrate 12ml 4°C (Protect from light)
ARG80246-010 STOP solution 12ml 4°C
ARG80246-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80246 Rat CCL2 / MCP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CCL2 / MCP1 in serum, plasma and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Mouse CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL5/RANTES, CCL6/C10, CCL7/MCP-3/MARC, CCL11/Eotaxin, CCL12/MCP-5, CXCL1/KC, CXCL2/MIP-2, G-CSF, GM-CSF, IFN- gamma, IL-1 alpha,1 beta,2,3,4,5,6;human CCL2/MCP-1, CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL5/RANTES, CCL7/MCP-3, CCL8/MCP-2
Target Name CCL2 / MCP1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24770 Rat CCL2

Swiss-port # P14844 Rat C-C motif chemokine 2

Gene Symbol Ccl2
Gene Full Name chemokine (C-C motif) ligand 2
Background This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2008]
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
PTM Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Title Download Link
ARG80246 Rat MCP-1 / CCL2 ELISA Kit User's manual download icon Download