ARG82111

Rat CD130 / gp130 ELISA Kit

Rat CD130 / gp130 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82111-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82111-002 Standard 2 X 50 ng/vial 4°C
ARG82111-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82111-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82111-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82111-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82111-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82111-008 25X Wash buffer 20 ml 4°C
ARG82111-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82111-010 STOP solution 10 ml (Ready to use) 4°C
ARG82111-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82111 Rat CD130 / gp130 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CD130 / gp130 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD130 / gp130
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%; Inter-Assay CV: 7.0%
Alternate Names CDw130; CD130; CDW130; Interleukin-6 signal transducer; CD antigen CD130; IL-6RB; Membrane glycoprotein 130; GP130; Oncostatin-M receptor subunit alpha; IL-6R subunit beta; Interleukin-6 receptor subunit beta; gp130; IL-6 receptor subunit beta; IL-6R-beta

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL6ST
Gene Full Name interleukin 6 signal transducer
Background The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
Function Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt]
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CD130 antibodies; CD130 ELISA Kits;
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PTM Phosphorylation of Ser-782 down-regulates cell surface expression.

Heavily N-glycosylated (PubMed:11098061, PubMed:16335952, PubMed:19159218, PubMed:19139490, PubMed:11251120). Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding (PubMed:19915009). [UniProt]
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ARG82111 Rat CD130 / gp130 ELISA Kit User's manual download icon Download