ARG81722

Rat CD86 ELISA Kit

Rat CD86 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81722-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81722-002 Standard 2 X 10 ng/vial 4°C
ARG81722-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81722-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81722-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81722-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81722-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81722-008 25X Wash buffer 20 ml 4°C
ARG81722-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81722-010 STOP solution 10 ml (Ready to use) 4°C
ARG81722-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81722 Rat CD86 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CD86 in serum and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD86
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%; Inter-Assay CV: 7.2%
Alternate Names B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD86
Gene Full Name CD86 molecule
Background This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
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PTM Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. [UniProt]
Title Download Link
ARG81722 Rat CD86 ELISA Kit User's manual download icon Download