ARG82855

Rat CES3 ELISA Kit

Rat CES3 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82855-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82855-002 Standard 2 X 10 ng/vial 4°C
ARG82855-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82855-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82855-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82855-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82855-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82855-008 25X Wash buffer 20 ml 4°C
ARG82855-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82855-010 STOP solution 10 ml (Ready to use) 4°C
ARG82855-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82855 Rat CES3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CES3 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Target Name CES3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%
Inter-Assay CV: 6.4%
Alternate Names ES31; Liver carboxylesterase 31 homolog; Carboxylesterase 3; EC 3.1.1.1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CES3
Gene Full Name carboxylesterase 3
Background This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined. [provided by RefSeq, Jun 2010]
Function Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows low catalytic efficiency for hydrolysis of CPT-11 (7-ethyl-10-[4-(1-piperidino)-1-piperidino]-carbonyloxycamptothecin), a prodrug for camptothecin used in cancer therapeutics. [UniProt]
Cellular Localization Endoplasmic reticulum lumen. [UniProt]
PTM N-glycosylated. [UniProt]
Title Download Link
ARG82855 Rat CES3 ELISA Kit User manual download icon Download