ARG82855
Rat CES3 ELISA Kit
Rat CES3 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82855-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82855-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82855-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82855-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82855-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82855-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82855-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82855-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82855-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82855-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82855-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82855 Rat CES3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CES3 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | CES3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 6.4% |
| Alternate Names | ES31; Liver carboxylesterase 31 homolog; Carboxylesterase 3; EC 3.1.1.1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CES3 |
|---|---|
| Gene Full Name | carboxylesterase 3 |
| Background | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined. [provided by RefSeq, Jun 2010] |
| Function | Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows low catalytic efficiency for hydrolysis of CPT-11 (7-ethyl-10-[4-(1-piperidino)-1-piperidino]-carbonyloxycamptothecin), a prodrug for camptothecin used in cancer therapeutics. [UniProt] |
| Cellular Localization | Endoplasmic reticulum lumen. [UniProt] |
| PTM | N-glycosylated. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82855 Rat CES3 ELISA Kit User manual |
 Download
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