ARG82414
Rat CPE / Carboxypeptidase E ELISA Kit
Rat CPE / Carboxypeptidase E ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82414-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82414-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82414-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82414-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82414-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82414-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82414-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82414-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82414-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82414-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82414-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82414 Rat CPE / Carboxypeptidase E ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CPE / Carboxypeptidase E in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | CPE / Carboxypeptidase E |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9% Inter-Assay CV: 6.3% |
| Alternate Names | CPH; Carboxypeptidase H; Enkephalin convertase; CPE; Carboxypeptidase E; Prohormone-processing carboxypeptidase; EC 3.4.17.10 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CPE |
| Gene Full Name | carboxypeptidase E |
| Background | This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq, Jul 2008] |
| Function | Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. [UniProt] |
| Cellular Localization | Isoform 1: Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted. Note=Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions. Isoform 2: Nucleus. [UniProt] |
| Highlight | Related products: Carboxypeptidase antibodies; Carboxypeptidase ELISA Kits; Carboxypeptidase recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG82414 Rat CPE / Carboxypeptidase E ELISA kit User manual |
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