ARG82379
Rat CXADR / CAR ELISA Kit
Rat CXADR / CAR ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82379-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82379-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82379-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82379-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82379-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82379-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82379-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82379-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82379-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82379-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82379-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82379 Rat CXADR / CAR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CXADR / CAR in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | CXADR / CAR |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 7.2% |
| Alternate Names | Coxsackievirus B-adenovirus receptor; CAR4/6; HCAR; hCAR; CAR; Coxsackievirus and adenovirus receptor; CVB3-binding protein; HCVADR |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9R066 Rat Coxsackievirus and adenovirus receptor homolog |
|---|---|
| Gene Symbol | CXADR |
| Gene Full Name | coxsackie virus and adenovirus receptor |
| Background | The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011] |
| Function | Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell junction, adherens junction. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions (PubMed:12297051). In airway epithelial cells localized to basolateral membrane but not to apical surface (PubMed:11316797). Isoform 3: Secreted. Isoform 4: Secreted. Isoform 5: Secreted. [UniProt] |
| Highlight | Related products: CXADR antibodies; CXADR ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82379 Rat CXADR / CAR ELISA Kit User manual |
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