ARG81908

Rat CXCL3 / GRO gamma ELISA Kit

Rat CXCL3 / GRO gamma ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81908-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81908-002 Standard 2 X 10 ng/vial 4°C
ARG81908-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81908-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81908-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81908-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81908-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81908-008 25X Wash buffer 20 ml 4°C
ARG81908-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81908-010 STOP solution 10 ml (Ready to use) 4°C
ARG81908-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81908 Rat CXCL3 / GRO gamma ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CXCL3 / GRO gamma in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CXCL3 / GRO gamma
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.6%; Inter-Assay CV: 7.4%
Alternate Names Macrophage inflammatory protein 2-beta; MIP2-beta; SCYB3; GRO-gamma; Growth-regulated protein gamma; 5-73; GROg; 1-73; MIP2B; C-X-C motif chemokine 3; CINC-2b; GRO3; MIP-2b

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 171551 Rat CXCL3

Swiss-port # Q10746 Rat C-X-C motif chemokine 3

Gene Symbol CXCL3
Gene Full Name chemokine (C-X-C motif) ligand 3
Background This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]
Function Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. [UniProt]
Highlight Related products:
CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins;
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Simplify the ELISA analysis by GainData
PTM N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt]
Title Download Link
ARG81908 Rat CXCL3 / GRO gamma ELISA Kit User's manual download icon Download