ARG81992

Rat CXCL4 / PF4 ELISA Kit

Rat CXCL4 / PF4 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81992-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81992-002 Standard 2 X 10 ng/vial 4°C
ARG81992-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81992-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81992-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81992-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81992-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81992-008 25X Wash buffer 20 ml 4°C
ARG81992-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81992-010 STOP solution 10 ml (Ready to use) 4°C
ARG81992-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81992 Rat CXCL4 / PF4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CXCL4 / PF4 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CXCL4 / PF4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%; Inter-Assay CV: 6.1%
Alternate Names Platelet factor 4; Iroplact; CXCL4; C-X-C motif chemokine 4; Oncostatin-A; SCYB4; PF-4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 360918 Rat PF4

Swiss-port # P06765 Rat Platelet factor 4

Gene Symbol PF4
Gene Full Name platelet factor 4
Background This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014]
Function Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. [UniProt]
Highlight Related products:
CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins;
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Simplify the ELISA analysis by GainData
Title Download Link
ARG81992 Rat CXCL4 / PF4 ELISA Kit User manual download icon Download