ARG82124
Rat Cardiac Troponin I ELISA Kit
Rat Cardiac Troponin I ELISA Kit for ELISA and Rat
Overview
| Product Description | ARG82124 Rat Cardiac Troponin I ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Cardiac Troponin I in serum and plasma. |
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| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | The following recombinant Rat proteins were tested and exhibited no cross-reactivity or interference: Adiponectin, ApoAI, BMP1, BMP2, BMP3, BMP4, BMP5, BMP7, CCL2, CCL4, CCL5, CRP, HGF, HSP27, IL1 beta, IL1RA, IL1 alpha, IL1 RI, IL2, IL4, IL5, IL6, IL6R, IL8, IL12, IL13, IL15, IL17C, IL21, IL23, IFN beta, IFN gamma, PDGF, PLA2G7, prolactin, serpin E1, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TL3, TLR9, TNF alpha, TNF RI, TNF RII, VEGF, VEGF R1. |
| Target Name | Cardiac Troponin I |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 0.15 ng/ml |
| Sample Type | Serum and plasma. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5% Inter-Assay CV: 8% |
| Alternate Names | RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7 |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | TNNI3 |
| Gene Full Name | troponin I type 3 (cardiac) |
| Background | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008] |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt] |
| Highlight | Related products: Troponin antibodies; Troponin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). [UniProt] |
| Title | Download Link |
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| ARG82124 Rat Cardiac Troponin I ELISA Kit User manual |
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