ARG82348
Rat DKK1 ELISA Kit
Rat DKK1 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82348-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82348-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82348-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82348-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82348-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82348-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82348-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82348-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82348-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82348-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82348-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82348 Rat DKK1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat DKK1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | DKK1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 23.45 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 46.9 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.5% Inter-Assay CV: 9.0% |
| Alternate Names | Dkk-1; Dickkopf-1; SK; DKK-1; hDkk-1; Dickkopf-related protein 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | DKK1 |
|---|---|
| Gene Full Name | dickkopf WNT signaling pathway inhibitor 1 |
| Background | This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008] |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: DKK1 antibodies; DKK1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG82348 Rat DKK1 ELISA Kit User manual |
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