ARG81910
Rat Endostatin ELISA Kit
Rat Endostatin ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81910-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81910-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81910-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81910-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81910-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81910-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81910-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81910-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81910-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81910-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81910-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81910 Rat Endostatin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Endostatin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Endostatin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.7% |
| Alternate Names | KS; KNO; KNO1; Collagen alpha-1(XVIII) chain |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | COL18A1 |
|---|---|
| Gene Full Name | collagen, type XVIII, alpha 1 |
| Background | This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Function | COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. [UniProt] |
| Highlight | Related products: Endostatin antibodies; Endostatin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Prolines at the third position of the tripeptide repeating unit (G-X-Y) of the triple-helical regions are hydroxylated. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81910 Rat Endostatin ELISA Kit User manual |
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