ARG82336

Rat Endothelin 1 ELISA Kit

Rat Endothelin 1 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82336-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82336-002 Standard 2 X 1 ng/vial 4°C
ARG82336-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82336-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82336-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82336-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82336-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82336-008 25X Wash buffer 20 ml 4°C
ARG82336-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82336-010 STOP solution 10 ml (Ready to use) 4°C
ARG82336-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82336 Rat Endothelin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Endothelin 1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Target Name Endothelin 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 1.95 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 3.9 - 250 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.0%
Inter-Assay CV: 7.3%
Alternate Names HDLCQ7; ET1; PPET1; ET-1; Preproendothelin-1; ARCND3; Endothelin-1; QME

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24323 Rat EDN1

Swiss-port # P22388 Rat Endothelin-1

Gene Symbol EDN1
Gene Full Name endothelin 1
Background The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]
Function Endothelins are endothelium-derived vasoconstrictor peptides. [UniProt]
Cellular Localization Secreted. [UniProt]
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Title Download Link
ARG82336 Rat Endothelin 1 ELISA Kit User manual download icon Download