ARG81829
Rat FGF acidic ELISA Kit
Rat FGF acidic ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81829-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81829-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81829-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81829-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81829-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81829-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81829-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81829-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81829-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81829-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81829-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81829 Rat FGF acidic ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat FGF acidic in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FGF acidic |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3%; Inter-Assay CV: 7.4% |
| Alternate Names | HBGF1; FGF-1; FGF-alpha; GLIO703; ECGFA; ECGFB; ECGF; Endothelial cell growth factor; FGFA; Acidic fibroblast growth factor; Heparin-binding growth factor 1; Fibroblast growth factor 1; HBGF-1; AFGF; aFGF; ECGF-beta |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF1 |
| Gene Full Name | fibroblast growth factor 1 (acidic) |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009] |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt] |
| Highlight | Related products: FGF acidic antibodies; FGF acidic ELISA Kits; FGF acidic recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | In the nucleus, phosphorylated by PKC/PRKCD. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81829 Rat FGF acidic ELISA Kit User manual |
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