ARG82495

Rat FLT3 Ligand ELISA Kit

Rat FLT3 Ligand ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82495-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82495-002 Standard 2 X 10 ng/vial 4°C
ARG82495-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82495-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82495-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82495-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82495-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82495-008 25X Wash buffer 20 ml 4°C
ARG82495-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82495-010 STOP solution 10 ml (Ready to use) 4°C
ARG82495-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82495 Rat FLT3 Ligand ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat FLT3 Ligand in serum and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Target Name FLT3 Ligand
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.3%
Inter-Assay CV: 6.1%
Alternate Names FLT3L; SL cytokine; Fms-related tyrosine kinase 3 ligand; Flt3L; Flt3 ligand; FL

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol FLT3LG
Gene Full Name fms-related tyrosine kinase 3 ligand
Background Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]
Function Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt]
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Title Download Link
ARG82495 Rat FLT3 Ligand ELISA Kit User manual download icon Download