ARG81644

Rat Fibronectin ELISA Kit

Rat Fibronectin ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81644-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81644-002 Standard 2 X 10 ng/vial 4°C
ARG81644-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81644-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81644-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81644-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81644-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81644-008 25X Wash buffer 20 ml 4°C
ARG81644-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81644-010 STOP solution 10 ml (Ready to use) 4°C
ARG81644-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81644 Rat Fibronectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Fibronectin in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Fibronectin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%; Inter-Assay CV: 6.4%
Alternate Names ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol FN1
Gene Full Name fibronectin 1
Background This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt]
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PTM Sulfated.

It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.

Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).

Phosphorylated by FAM20C in the extracellular medium.

Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. [UniProt]
Title Download Link
ARG81644 Rat Fibronectin ELISA Kit User manual download icon Download