ARG82114
Rat GAS6 ELISA Kit
Rat GAS6 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82114-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82114-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82114-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82114-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82114-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82114-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82114-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82114-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82114-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82114-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82114-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82114 Rat GAS6 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat GAS6 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | GAS6 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.1%; Inter-Assay CV: 6.7% |
| Alternate Names | Growth arrest-specific protein 6; AXLLG; AXSF; GAS-6; AXL receptor tyrosine kinase ligand |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GAS6 |
|---|---|
| Gene Full Name | growth arrest-specific 6 |
| Background | This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014] |
| Function | Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: GAS6 antibodies; GAS6 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82114 Rat GAS6 ELISA Kit User's manual |
 Download
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