ARG82103

Rat Galectin 1 ELISA Kit

Rat Galectin 1 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82103-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82103-002 Standard 2 X 10 ng/vial 4°C
ARG82103-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82103-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82103-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82103-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82103-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82103-008 25X Wash buffer 20 ml 4°C
ARG82103-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82103-010 STOP solution 10 ml (Ready to use) 4°C
ARG82103-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82103 Rat Galectin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Galectin 1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Galectin 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 7.2%
Alternate Names HBL; Gal-1; GAL1; HPL; Lectin galactoside-binding soluble 1; Galectin-1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; GBP; Beta-galactoside-binding lectin L-14-I; Putative MAPK-activating protein PM12; 14 kDa lectin; Galaptin; S-Lac lectin 1; HLBP14

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56646 Rat LGALS1

Swiss-port # P11762 Rat Galectin-1

Gene Symbol LGALS1
Gene Full Name lectin, galactoside-binding, soluble, 1
Background The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]
Function May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
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Title Download Link
ARG82103 Rat Galectin 1 ELISA Kit User's manual download icon Download