ARG81879

Rat HGF ELISA Kit

Rat HGF ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81879-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81879-002 Standard 2 X 10 ng/vial 4°C
ARG81879-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81879-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81879-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81879-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81879-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81879-008 25X Wash buffer 20 ml 4°C
ARG81879-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81879-010 STOP solution 10 ml (Ready to use) 4°C
ARG81879-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81879 Rat HGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat HGF in serum, plasma (EDTA, citrate) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name HGF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, citrate) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.0%; Inter-Assay CV: 8.0%
Alternate Names HPTA; Scatter factor; F-TCF; Hepatocyte growth factor; DFNB39; Hepatopoietin-A; HGFB; SF

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24446 Rat HGF

Swiss-port # P17945 Rat Hepatocyte growth factor

Gene Symbol HGF
Gene Full Name hepatocyte growth factor (hepapoietin A; scatter factor)
Background Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. [UniProt]
Highlight Related products:
HGF antibodies; HGF ELISA Kits; HGF recombinant proteins;
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Title Download Link
ARG81879 Rat HGF ELISA Kit User manual download icon Download