ARG70313
Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter)
Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Rat
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Rat IFN gamma recombinant protein. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | Binding, SDS-PAGE |
| Target Name | IFN gamma |
| Species | Rat |
| A.A. Sequence | Gln23 - Cys156 of Rat IFN gamma (NP_620235.1) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IFN-gamma R1 at 3µg/ml (100µl/well) can bind recombinant Rat IFNG, the EC50 of Rat IFNG is 1 µg/ml. |
|---|
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 92% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IFNG |
|---|---|
| Gene Full Name | interferon, gamma |
| Background | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015] |
| Function | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 19 kDa |
| PTM | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. [UniProt] |
