ARG80237

Rat IL1 alpha ELISA Kit

Rat IL1 alpha ELISA Kit for ELISA and Rat

Immune System kit; Metabolism kit

Component

Cat No Component Name Package Temp
ARG80237-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80237-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80237-003 Standard diluent buffer 20 ml 4°C
ARG80237-004 Antibody conjugate concentrate 400 µl 4°C
ARG80237-005 Antibody diluent buffer 16 ml 4°C
ARG80237-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80237-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80237-008 20X Wash buffer 50 ml 4°C
ARG80237-009 TMB substrate 12ml 4°C (Protect from light)
ARG80237-010 STOP solution 12ml 4°C
ARG80237-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80237 Rat IL1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL1 alpha in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-1 alpha, IL-18, IL-1 beta;mouse IL-1 alpha, IL-18, IL-1 beta;rat IL-18, IL-1 beta;porcine IL-1 alpha, IL-1 beta
Target Name IL1 alpha
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 4 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision CV: <10%
Alternate Names IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24493 Rat IL1A

Swiss-port # P16598 Rat Interleukin-1 alpha

Gene Symbol Il1a
Gene Full Name interleukin 1 alpha
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq]
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Research Area Immune System kit; Metabolism kit
Title Download Link
ARG80237 Rat IL1 alpha ELISA Kit User's manual download icon Download