ARG80235

Rat IL1 beta ELISA Kit

Rat IL1 beta ELISA Kit for ELISA and Rat

Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit

Component

Cat No Component Name Package Temp
ARG80235-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80235-002 Standard (Lyophilized) 3 X 3.2 ng/vial 4°C
ARG80235-003 Standard diluent buffer 20 ml 4°C
ARG80235-004 Antibody conjugate concentrate 400 µl 4°C
ARG80235-005 Antibody diluent buffer 16 ml 4°C
ARG80235-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80235-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80235-008 20X Wash buffer 50 ml 4°C
ARG80235-009 TMB substrate 12ml 4°C (Protect from light)
ARG80235-010 STOP solution 12ml 4°C
ARG80235-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80235 Rat IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL1 beta in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-2,4,1 alpha,sIL-2R,sIL-4R;mouse IL-2,4,10,1 alpha;rat GM-CSF, IL-2,4,10,1 alpha, SCF, TNF- alpha
Target Name IL1 beta
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 30 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol Il1b
Gene Full Name interleukin 1 beta
Background IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
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Research Area Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
Title Download Link
ARG80235 Rat IL1 beta ELISA Kit User's manual download icon Download