ARG81993

Rat IL12 p70 ELISA Kit

Rat IL12 p70 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81993-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81993-002 Standard 2 X 10 ng/vial 4°C
ARG81993-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81993-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81993-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81993-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81993-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81993-008 25X Wash buffer 20 ml 4°C
ARG81993-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81993-010 STOP solution 10 ml (Ready to use) 4°C
ARG81993-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81993 Rat IL12 p70 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL12 p70 in serum and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL12 p70
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.0%; Inter-Assay CV: 7.1%
Alternate Names CLMF; Cytotoxic lymphocyte maturation factor 35 kDa subunit; NFSK; NK cell stimulatory factor chain 1; CLMF p35; NKSF1; IL-12 subunit p35; IL-12A; Interleukin-12 subunit alpha; P35

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 84405 Rat IL12A

Swiss-port # Q9R103 Rat Interleukin-12 subunit alpha

Gene Symbol IL12A
Gene Full Name interleukin 12A
Background This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. [UniProt]
Highlight Related products:
IL12 p70 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG81993 Rat IL12 p70 ELISA Kit User's manual download icon Download