ARG81774

Rat IL13 ELISA Kit

Rat IL13 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81774-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81774-002 Standard 2 X 10 ng/vial 4°C
ARG81774-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81774-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81774-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81774-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81774-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81774-008 25X Wash buffer 20 ml 4°C
ARG81774-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81774-010 STOP solution 10 ml (Ready to use) 4°C
ARG81774-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81774 Rat IL13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL13 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL13
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%; Inter-Assay CV: 7.8%
Alternate Names Interleukin-13; P600; IL-13

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 116553 Rat IL13

Swiss-port # P42203 Rat Interleukin-13

Gene Symbol IL13
Gene Full Name interleukin 13
Background This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]
Function Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. [UniProt]
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IL13 antibodies; IL13 ELISA Kits; IL13 recombinant proteins;
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Title Download Link
ARG81774 Rat IL13 ELISA Kit User's manual download icon Download