ARG81692

Rat IL18 ELISA Kit

Rat IL18 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81692-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81692-002 Standard 2 X 10 ng/vial 4°C
ARG81692-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81692-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81692-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81692-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81692-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81692-008 25X Wash buffer 20 ml 4°C
ARG81692-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81692-010 STOP solution 10 ml (Ready to use) 4°C
ARG81692-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81692 Rat IL18 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL18 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL18
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%; Inter-Assay CV: 6.4%
Alternate Names Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29197 Rat IL18

Swiss-port # P97636 Rat Interleukin-18

Gene Symbol IL18
Gene Full Name interleukin 18
Background The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt]
Highlight Related products:
IL18 antibodies; IL18 ELISA Kits; IL18 recombinant proteins;
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Simplify the ELISA analysis by GainData
PTM The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt]
Title Download Link
ARG81692 Rat IL18 ELISA Kit User's manual download icon Download