ARG80232

Rat IL4 ELISA Kit

Rat IL4 ELISA Kit for ELISA and Rat

Cancer kit; Immune System kit

Component

Cat No Component Name Package Temp
ARG80232-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80232-002 Standard (Lyophilized) 3 X 4 ng/vial 4°C
ARG80232-003 Standard diluent buffer 20 ml 4°C
ARG80232-004 Antibody conjugate concentrate 400 µl 4°C
ARG80232-005 Antibody diluent buffer 16 ml 4°C
ARG80232-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80232-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80232-008 20X Wash buffer 50 ml 4°C
ARG80232-009 TMB substrate 12ml 4°C (Protect from light)
ARG80232-010 STOP solution 12ml 4°C
ARG80232-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80232 Rat IL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL4 in serum, plasma and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-1 sRI, IL-4, IL-7 R alpha, GDNF;mouse IL-4
Target Name IL4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision CV: <10%
Alternate Names BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 287287 Rat IL4

Swiss-port # P20096 Rat Interleukin-4

Gene Symbol Il4
Gene Full Name interleukin 4
Background This protein is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Highlight Related products:
IL4 antibodies; IL4 ELISA Kits; IL4 recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cancer kit; Immune System kit
Title Download Link
ARG80232 Rat IL4 ELISA Kit User's manual download icon Download