ARG80239

Rat IgG1 ELISA Kit

Rat IgG1 ELISA Kit for ELISA and Rat

Immune System kit

Component

Cat No Component Name Package Temp
ARG80239-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80239-002 Standard (Lyophilized) 3 X 250 ng/vial 4°C
ARG80239-003 Standard diluent buffer 20 ml 4°C
ARG80239-004 Antibody conjugate concentrate 400 µl 4°C
ARG80239-005 Antibody diluent buffer 16 ml 4°C
ARG80239-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80239-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80239-008 20X Wash buffer 50 ml 4°C
ARG80239-009 TMB substrate 12ml 4°C (Protect from light)
ARG80239-010 STOP solution 12ml 4°C
ARG80239-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80239 Rat IgG1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IgG1 in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Rat
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Rat IgG2, IgG3 and IgG4
Target Name IgG1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.8 ng/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 1.56 - 100 ng/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names Immunoglobulin G1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background The IgG subclasses contain antibodies to components of many disease-causing bacteria and viruses, each subclass serves a slightly different function in protecting the body against infection. For example, IgG1 and IgG3 subclasses are rich in antibodies against proteins such as the toxins produced by the diphtheria and tetanus bacteria, as well as antibodies against viral proteins. In contrast, IgG2 antibodies are predominantly against the polysaccharide (complex sugar) coating (capsule) of certain disease-producing bacteria (such as, Streptococcus pneumoniae and Haemophilus influenzae).
Highlight Related products:
IgG1 ELISA Kits; IgG1 recombinant proteins;
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Simplify the ELISA analysis by GainData
Research Area Immune System kit
Title Download Link
ARG80239 Rat IgG1 ELISA Kit User's manual download icon Download