ARG81693

Rat MMP2 ELISA Kit

Rat MMP2 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81693-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81693-002 Standard 2 X 40 ng/vial 4°C
ARG81693-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81693-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81693-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81693-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81693-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81693-008 25X Wash buffer 20 ml 4°C
ARG81693-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81693-010 STOP solution 10 ml (Ready to use) 4°C
ARG81693-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81693 Rat MMP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat MMP2 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MMP2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 312.5 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 625 - 40000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.5%
Alternate Names CLG4A; MMP-2; TBE-1; MONA; CLG4; EC 3.4.24.24; Gelatinase A; Matrix metalloproteinase-2; MMP-II; 72 kDa gelatinase; 72 kDa type IV collagenase

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 81686 Rat MMP2

Swiss-port # P33436 Rat 72 kDa type IV collagenase

Gene Symbol MMP2
Gene Full Name matrix metallopeptidase 2
Background This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Function Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.

PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.

Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. [UniProt]
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PTM Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.

The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. [UniProt]
Title Download Link
ARG81693 Rat MMP2 ELISA Kit User's manual download icon Download