ARG82364

Rat MSTN / GDF8 ELISA Kit

Rat MSTN / GDF8 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82364-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82364-002 Standard 2 X 10 ng/vial 4°C
ARG82364-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82364-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82364-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82364-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82364-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82364-008 25X Wash buffer 20 ml 4°C
ARG82364-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82364-010 STOP solution 10 ml (Ready to use) 4°C
ARG82364-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82364 Rat MSTN / GDF8 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat MSTN / GDF8 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Target Name MSTN / GDF8
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.0%
Inter-Assay CV: 6.3%
Alternate Names GDF-8; Myostatin; GDF8; MSLHP; Growth/differentiation factor 8

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29152 Rat MSTN

Swiss-port # O35312 Rat Growth/differentiation factor 8

Gene Symbol MSTN
Gene Full Name myostatin
Background The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. [provided by RefSeq, Jul 2008]
Function Acts specifically as a negative regulator of skeletal muscle growth. [UniProt]
Cellular Localization Secreted. [UniProt]
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Title Download Link
ARG82364 Rat MSTN / GDF8 ELISA kit User manual download icon Download